云南籍正常人群和肺癌人群GSTM1基因型多态性的比较研究
|
摘要
实验采用聚合酶链式反应 ( Polymerase Chain Reaction,PCR)技术对云南籍正常人群和肺癌人群的 GSTM1基因型多态性进行了检测。GSTM1基因纯合缺失 ( GSTM1 0 /0 )在正常人群中的分布频率为 65 .8% ( n =99) ,而在肺癌人群中分布频率为 76.8% ( n =5 6)。结果表明 GSTM1基因型多态性在云南籍正常人群和肺癌人群中的分布频率无显著性差异。本研究为进一步寻找云南人群肺癌遗传易感因子奠定了基础
The Polymerase Chain Reaction (PCR) was employed in this experiment to compare the genetic polymorphism of GSTM1 gene between normal population and lung cancer population in Yunnan province. The frequencies of GSTM1 null genotype (GSTM10/0) were 65.8 % in normal population and were 76.8% in lung cancer population. No significant differences were found in the GSTM1 genotype frequencies among two groups (P>0.05). i.e., The result is helpful for further research on the genetic susceptibility factor of lung cancer.
The Polymerase Chain Reaction (PCR) was employed in this experiment to compare the genetic polymorphism of GSTM1 gene between normal population and lung cancer population in Yunnan province. The frequencies of GSTM1 null genotype (GSTM10/0) were 65.8 % in normal population and were 76.8% in lung cancer population. No significant differences were found in the GSTM1 genotype frequencies among two groups (P>0.05). i.e., The result is helpful for further research on the genetic susceptibility factor of lung cancer.
引文
[1] W.AuWilliam.HyeYoungOh et al..Usefulness ofGeneticSusceptibility andBiomarkers forEvaluation ofEn-vironmentalHealthRisk[J].Environmental andMolecularMutagenesis.2001,37:215-225.
[2] A.Hirvonen.Husgafvel-PursiainenK et al..TheGSTM1 null genotype as a potential risk modifier for squa-mous cell carcinoma of the lung[J].Carcinogenesis.1993,17:1479-1481.
[3] J.Salagovic.I.Kalina et al.Genetic polymorphic of glutathioneS-transferasesM1 andT1 as a risk factor inlung and bladder cancers[J].IntJCancer.1998,77:312-317.
[4] Y.S.Hong.J.H.Chang.Polymorphism of theCYP1A1 and glutathione-S-transferase gene inKorean lungcancer patients[J].NatGenet.1998;3:192-198.
[5] Persson.I.Johansson etal..Genetic polymorphism of xenobiotic metabolizing enzymes amongChinese lung can-cer patients[J].IntJCancer.1999,81:325-329.
[6] S.Zhong.A.H.Wyllie.Relationship between theGSTM1 genetic polymorphism and susceptibility to bladder,breast and colon cancer[J].Carcinogenesis.1993,14(9):1821-1824.
[7] E.Z.Randa.B.Joseph etal..Combined genetic polymorphism and risk for developmentof lung cancer.[J].Mu-tationResearch.1997,38:189-200.
[8] 林国芳,谭靖伟,沈建花.上海正常人群中古胱甘肽S-转移酶T1基因纯合缺损[J].癌变·畸变·突变.1998,10(6):345-348.
[9] 林国芳,谭靖伟,沈建华.上海“本地人”正常人群GSTM1和GSTT1基因型多态性研究[J].癌变·畸变·突变.2001,13(1):10-12.
[10] 高坚瑞,张桥.GSTM1多态性与肺癌敏感性关系研究[J].癌变·畸变·突变.1998,10(3):149-151.
[11] J.Seidegard.R.W.Pero.The hereditary transmission of high glutathione transferase activity towards trans-stilbene oxide in human mononuclear leucocytes[J].Hum.Genet.1985,69:66-68
[2] A.Hirvonen.Husgafvel-PursiainenK et al..TheGSTM1 null genotype as a potential risk modifier for squa-mous cell carcinoma of the lung[J].Carcinogenesis.1993,17:1479-1481.
[3] J.Salagovic.I.Kalina et al.Genetic polymorphic of glutathioneS-transferasesM1 andT1 as a risk factor inlung and bladder cancers[J].IntJCancer.1998,77:312-317.
[4] Y.S.Hong.J.H.Chang.Polymorphism of theCYP1A1 and glutathione-S-transferase gene inKorean lungcancer patients[J].NatGenet.1998;3:192-198.
[5] Persson.I.Johansson etal..Genetic polymorphism of xenobiotic metabolizing enzymes amongChinese lung can-cer patients[J].IntJCancer.1999,81:325-329.
[6] S.Zhong.A.H.Wyllie.Relationship between theGSTM1 genetic polymorphism and susceptibility to bladder,breast and colon cancer[J].Carcinogenesis.1993,14(9):1821-1824.
[7] E.Z.Randa.B.Joseph etal..Combined genetic polymorphism and risk for developmentof lung cancer.[J].Mu-tationResearch.1997,38:189-200.
[8] 林国芳,谭靖伟,沈建花.上海正常人群中古胱甘肽S-转移酶T1基因纯合缺损[J].癌变·畸变·突变.1998,10(6):345-348.
[9] 林国芳,谭靖伟,沈建华.上海“本地人”正常人群GSTM1和GSTT1基因型多态性研究[J].癌变·畸变·突变.2001,13(1):10-12.
[10] 高坚瑞,张桥.GSTM1多态性与肺癌敏感性关系研究[J].癌变·畸变·突变.1998,10(3):149-151.
[11] J.Seidegard.R.W.Pero.The hereditary transmission of high glutathione transferase activity towards trans-stilbene oxide in human mononuclear leucocytes[J].Hum.Genet.1985,69:66-68
目录