Astrocyte-targeted production of IL-10 induces changes in microglial reactivity and reduces motor neuron death after facial nerve axotomy

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• 作者：Nà ; dia Villacampa ; Beatriz Almolda ; Antonietta Vilella ; Iain L. Campbell ; Berta Gonzá ; lez and Bernardo Castellano
• 关键词：microglia ; transgenic animal ; neuronal survival ; lymphocytes ; nerve injury ; neurodegeneration ; cytokines ; IL-10R ; CD18 ; CD16/32 ; MHC-II ; CD39 ; arginase-1 ; Ym-1 ; CD150
• 刊名：Glia
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：63
• 期：7
• 页码：1166-1184
• 全文大小：2377K
• ISSN：1098-1136

文摘

Interleukin-10 (IL-10) is a cytokine that plays a crucial role in regulating the inflammatory response and immune reactions. In the central nervous system (CNS), IL-10 is mainly produced by astrocytes and microglia and it is upregulated after various insults, such as experimental autoimmune encephalomyelitis, middle cerebral artery occlusion, excitotoxicity and traumatic brain injury. To better understand the effects of IL-10 in the normal and injured CNS, we generated transgenic mice (termed GFAP-IL-10Tg) that expressed the murine IL-10 gene under the transcriptional control of the glial fibrillary acidic protein (GFAP) promoter. Previous studies demonstrated marked changes in the microglial phenotype in these mice under basal conditions. The objective of the present study was to investigate the effects of local astrocyte-targeted IL-10 production on glial activation, neuronal degeneration and leukocyte recruitment after axotomy. GFAP-IL-10Tg mice had marked changes in the phenotype of activated microglial cells, as well as in the number of microglial clusters and in microglial cell density. These microglial changes are accompanied by a twofold increase in lymphocyte infiltration in GFAP-IL-10Tg mice and around twofold decrease in neuronal cell death at 21 dpi. Altogether, our findings suggested that astrocyte-targeted production of IL-10 impacted the microglial response and lymphocyte recruitment and culminated in a beneficial effect on neuronal survival. GLIA 2015;63:1166–1184