Contribution of the coelomic epithelial cells specific to the left testis in the chicken embryo

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• 作者：Takuya Omotehara ; Kiichi Minami ; Youhei Mantani ; Yuria Umemura ; Miho Nishida ; Tetsushi Hirano ; Hidefumi Yoshioka ; Hiroshi Kitagawa ; Toshifumi Yokoyama and Nobuhiko Hoshi
• 刊名：Developmental Dynamics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：246
• 期：2
• 页码：148-156
• 全文大小：1100K
• ISSN：1097-0177

文摘

rlined ">Background: The left male gonad in the chicken embryo has a thickened cortical layer, but it eventually becomes flattened after the onset of testicular development. Because the destination of the cortical cells migrating from the left gonad remains unclear, we examined this issue herein. rlined ">Results: The testis-inducing gene doublesex- and mab-3-related transcription factor 1 (DMRT1) was detected in a proportion of the columnar and cubic epithelial cells in the cortex of the left testis as well as Sertoli cells in both testes. Interestingly, some of the DMRT1-expressing cortical cells were contiguous with Sertoli cells in the testis cord. Some cortical cells exhibited a vimentin-positive cytoplasm that was elongated all the way to the medulla. In addition, a desmosome-like structure was observed between the elongated cytoplasm in these cells and the adjacent Sertoli cell. After the organ culture, a few cells labeled with a fluorescent dye that stained only the cortical cells at the beginning of the culture were located in the testis cord of the left testis. rlined ">Conclusions: Some cortical cells expressing DMRT1 were suggested to contribute to the Sertoli cells in the testis cord only after the onset of testicular development and only in the left testis. Developmental Dynamics 246:148–156, 2017.