TET1-Mediated Oxidation of 5-Formylcytosine (5fC) to 5-Carboxycytosine (5caC) in RNA
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- 作者：Dr. Maria Basanta-Sanchez ; Dr. Rui Wang ; Zhenzhen Liu ; Xiaohan Ye ; Prof. Dr. Minyong Li ; Prof. Dr. Xiaodong Shi ; Prof. Dr. Paul F. Agris ; Prof. Dr. Yubin Zhou ; Prof. Dr. Yun Huang and Prof. Dr. Jia Sheng
- 刊名：ChemBioChem
- 出版年：2017
- 出版时间：January 3, 2017
- 年：2017
- 卷：18
- 期：1
- 页码：72-76
- 全文大小：563K
- ISSN：1439-7633
文摘
It was recently revealed that 5-methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), implying the potential regulatory roles of this post-transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5-carboxycytidine (5caC) by the catalytic domain of mammalian ten-eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single-stranded, double-stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.