Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging
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- 作者：Manuela A. H. Fichte ; Xenia M. M. Weyel ; Dr. Stephan Junek ; Dr. Florian Schfer ; Dr. Cyril Herbivo ; Prof. Dr. Maurice Goeldner ; Dr. Alexandre Specht ; Prof. Dr. Josef Wachtveitl and Prof. Dr. Alexander Heckel
- 刊名：Angewandte Chemie International Edition
- 出版年：2016
- 出版时间：July 25, 2016
- 年：2016
- 卷：55
- 期：31
- 页码：8948-8952
- 全文大小：2224K
- ISSN：1521-3773
文摘
We successfully introduced two-photon-sensitive photolabile groups ([7-(diethylamino)coumarin-4-yl]methyl and p-dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three-dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double-strand displacement in a hydrogel and in neurons. Orthogonal two-photon uncaging of the two cages is possible, thus enabling complex scenarios of three-dimensional control of hybridization with light.