Immobilization of a multi-enzyme system for L-amino acids production
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- 作者：Marí ; a J Rodrí ; guez-Alonso ; Felipe Rodrí ; guez-Vico ; Francisco J Las Heras-Vá ; zquez and Josefa M Clemente-Jimé ; nez
- 刊名：Journal of Chemical Technology & Biotechnology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：91
- 期：7
- 页码：1972-1981
- 全文大小：188K
- ISSN：1097-4660
文摘
Four enzymes were immobilized for the production of optically pure natural and non-natural L-amino acids via the ‘Double-Racemase Hydantoinase Process’. Immobilization constitutes an empirical process, and for each enzyme we tested 11 carriers with different functional groups; epoxide, EC-EP, EC-HFA, IB-150 and IB-350, carboxylic acid IB-C435, quaternary ammonium IB-A161, IB-A171 and IB-A369, aromatic group IB-S861, and hydroxyl group IB-S60S and IB-S60P.