Peptide backbone modification in the bend region of amyloid-β inhibits fibrillogenesis but not oligomer formation

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• 作者：Erik C. B. Johnson ; Jennifer D. Lanning and Stephen C. Meredith
• 刊名：Journal of Peptide Science
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：22
• 期：5
• 页码：368-373
• 全文大小：311K
• ISSN：1099-1387

文摘

Current evidence suggests that oligomers of the amyloid-β (Aβ) peptide are involved in the cellular toxicity of Alzheimer's disease, yet their biophysical characterization remains difficult because of lack of experimental control over the aggregation process under relevant physiologic conditions. Here, we show that modification of the Aβ peptide backbone at Gly29 allows for the formation of oligomers but inhibits fibril formation at physiologic temperature and pH. Our results suggest that the putative bend region in Aβ is important for higher-order aggregate formation. Copyright