文摘
A natural assemblage of microalgae from a facultative lagoon system treating municipal wastewater was enriched for growth in the effluents of an anaerobic digester processing dairy waste. A green microalga with close resemblance to Chlorella sp. was found to be dominant after multiple cycles of sub-culturing. Subsequently, the strain (designated as LLAI) was isolated and cultivated in 20× diluted digester effluents under various incident light intensities (255–1,100 µmoles m−2 s−1) to systematically assess growth and nutrient utilization. Our results showed that LLAI production increased with increasing incident light and a maximum productivity of 0.34 g L−1 d−1 was attained when the incident irradiance was 1,100 µmoles m−2 s−1. Lack of growth in the absence of light indicated that the cultures did not grow heterotrophically on the organic compounds present in the medium. However, the cultures were able to uptake organic N and P under phototrophic conditions and our calculations suggest that the carbon associated with these organic nutrients contributed significantly to the production of biomass. Overall, under high light conditions, LLAI cultures utilized half of the soluble organic nitrogen and >90% of the ammonium, orthophosphate, and dissolved organic phosphorus present in the diluted waste. Strain LLAI was also found to accumulate triacylglycerides (TAG) even before the onset of nutrient limitation and a lipid productivity of 37 mg-TAG L−1 d−1 was measured in cultures incubated at an incident irradiance of 1,100 µmoles m−2 s−1. The results of this study suggest that microalgae isolates from natural environments are well-suited for nutrient remediation and biomass production from wastewater containing diverse inorganic and organic nutrient species.