Detection of Helicobacter pylori by Real-Time PCR for 16s rRNA in Stools of NonInfected Healthy Children, Using ELISA Antigen Stool Test as the Gold Standard

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• 作者：Sergio George ; Nora Mamani ; Yalda Lucero ; Juan Pablo Torres ; Mauricio Farfá ; n ; Anne J Lagomarcino ; Andrea Orellana and Miguel O'Ryan
• 刊名：Helicobacter
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：21
• 期：6
• 页码：606-612
• 全文大小：620K
• ISSN：1523-5378

文摘

We previously detected Helicobacter pylori infection by stool antigen ELISA assay in 33-41% of asymptomatic Chilean children between 2–3 years of age, of which 11–20% had a transient infection and 21–22% a persistent infection. A total of 88% of ELISA-positive samples were also rtPCR positive, while 37/133 (33%) of ELISA-negative stool samples were rtPCR positive. The significance of a ELISA-negative/rtPCR-positive sample requires clarification. We aimed to determine whether rtPCR is able to detect persistent infections not detected by ELISA.