Synergistic influence of topomimetic and chondroitin sulfate-based treatments on osteogenic potential of Ti-6Al-4V
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- 作者：Bé ; atrice Labat ; Sandrine Morin-Grognet ; Fabien Gaudiè ; re ; Lucia Bertolini-Forno ; Olivier Thoumire ; Jean-Pierre Vannier ; Guy Ladam and Hassan Atmani
- 刊名：Journal of Biomedical Materials Research Part A
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：104
- 期：8
- 页码：1988-2000
- 全文大小：976K
- ISSN：1552-4965
文摘
We combined topographical and chemical surface modifications of Ti-6Al-4V (TA6V) to improve its osteogenic potential. By acid-etching, we first generated topomimetic surface features resembling, in size and roughness, bone cavities left by osteoclasts. Next, we coated these surfaces with biomimetic Layer-by-Layer films (LbL), composed of chondroitin sulfate A and poly-mallCaps">l-lysine that were mechanically tuned after a post-treatment with genipin. The structural impact of each surface processing step was thoroughly inspected. The desired nano/microrough topographies of TA6V were maintained upon LbL deposition. Whereas no significant promotion of adhesion and proliferation of MC3T3-E1 preosteoblasts were detected after independent or combined modifications of the topography and the chemical composition of the substrates, osteogenic maturation was promoted when both surface treatments were combined, as was evidenced by significant long-term matrix mineralization. The results open promising route toward improved osseointegration of titanium-based implants.