Analysis of 3D multi-layer microfluidic gradient generator

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• 作者：Jang Ho Ha ; Tae Hyeon Kim ; Jong Min Lee ; Christian D. Ahrberg and Bong Geun Chung
• 刊名：ELECTROPHORESIS
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：38
• 期：2
• 页码：270-277
• 全文大小：623K
• ISSN：1522-2683

文摘

We developed a three-dimensional (3D) simple multi-layer microfluidic gradient generator to create molecular gradients on the centimeter scale with a wide range of flow rates. To create the concentration gradients, a main channel (MC) was orthogonally intersected with vertical side microchannel (SC) in a 3D multi-layer microfluidic device. Through sequential dilution from the SC, a spatial gradient was generated in the MC. Two theoretical models were created to assist in the design of the 3D multi-layer microfluidic gradient generator and to compare its performance against a two-dimensional equivalent. A first mass balance model was used to predict the steady-state concentrations reached, while a second computational fluid dynamic model was employed to predict spatial development of the gradient by considering convective as well as diffusive mass transport. Furthermore, the theoretical simulations were verified through experiments to create molecular gradients in a 3D multi-layer microfluidic gradient generator.