DNA extraction from benthic Cyanobacteria: comparative assessment and optimization

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• 作者：V. Gaget ; A. Keulen ; M. Lau ; P. Monis and J.D. Brookes
• 刊名：Journal of Applied Microbiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：122
• 期：1
• 页码：294-304
• 全文大小：425K
• ISSN：1365-2672

文摘

Benthic Cyanobacteria produce toxic and odorous compounds similar to their planktonic counterparts, challenging the quality of drinking water supplies. The biofilm that benthic algae and other micro-organisms produce is a complex and protective matrix. Monitoring to determine the abundance and identification of Cyanobacteria, therefore, relies on molecular techniques, with the choice of DNA isolation technique critical. This study investigated which DNA extraction method is optimal for DNA recovery in order to guarantee the best DNA yield for PCR-based analysis of benthic Cyanobacteria.