Important role of phosphoramido linkage in imidazole-based dioleyl helper lipids for liposome stability and primary cell transfection
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- 作者：Mathieu Mé ; vel ; Thomas Haudebourg ; Thibault Colombani ; Pauline Peuziat ; Laurence Dallet ; Benoî ; t Chatin ; Olivier Lambert ; Mathieu Berchel ; Tristan Montier ; Paul-Alain Jaffrè ; s ; Pierre Lehn and Bruno Pitard
- 关键词：helper lipid ; liposome ; non viral vector ; primary cell ; transfection
- 刊名：The Journal of Gene Medicine
- 出版年：2016
- 出版时间：January-March 2016
- 年：2016
- 卷：18
- 期：1-3
- 页码：3-15
- 全文大小：457K
- ISSN：1521-2254
文摘
To optimize synthetic gene delivery systems, there is a need to develop more efficient lipid formulations. Most cationic lipid formulations contain ‘helper’ neutral lipids because of their ability to increase DNA delivery, in particular by improving endosomal escape of DNA molecules via the pH-buffering effect of protonatable groups and/or fusion with the lipid bilayer of endosomes.