Estimation of the diversity between DNA calorimetric profiles, differential melting curves and corresponding melting temperatures

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• 作者：Chun-Ling Chang ; Alexander S. Fridman ; Inessa E. Grigoryan ; Elena N. Galyuk ; Oleg N. Murashko ; Chin-Kun Hu and Dmitri Y. Lando
• 刊名：Biopolymers
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：105
• 期：11
• 页码：832-839
• 全文大小：789K
• ISSN：1097-0282

文摘

The Poland–Fixman–Freire formalism was adapted for modeling of calorimetric DNA melting profiles, and applied to plasmid pBR 322 and long random sequences. We studied the influence of the difference (H_GC−H_AT) between the helix-coil transition enthalpies of AT and GC base pairs on the calorimetric melting profile and on normalized calorimetric melting profile. A strong alteration of DNA calorimetrical profile with H_GC−H_AT was demonstrated. In contrast, there is a relatively slight change in the normalized profiles and in corresponding ordinary (optical) normalized differential melting curves (DMCs). For fixed H_GC−H_AT, the average relative deviation (S) between DMC and normalized calorimetric profile, and the difference between their melting temperatures (T_cal−T_m) are weakly dependent on peculiarities of the multipeak fine structure of DMCs. At the same time, both the deviation S and difference (T_cal−T_m) enlarge with the temperature melting range of the helix-coil transition. It is shown that the local deviation between DMC and normalized calorimetric profile increases in regions of narrow peaks distant from the melting temperature.