Near-Infrared Fluorescent Ag₂Se-Cetuximab Nanoprobes for Targeted Imaging and Therapy of Cancer

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• 作者：Chun-Nan Zhu ; Gang Chen ; Zhi-Quan Tian ; Wei Wang ; Wen-Qun Zhong ; Zheng Li ; Zhi-Ling Zhang and Dai-Wen Pang
• 刊名：Small
• 出版年：2017
• 出版时间：January 18, 2017
• 年：2017
• 卷：13
• 期：3
• 全文大小：4325K
• ISSN：1613-6829

文摘

Theranostic nanoprobes integrated with diagnostic imaging and therapy capabilities have shown great potential for highly effective tumor therapy by realizing imaging-guided drug delivery and tumor treatment. Developing novel high-performance nanoprobes is an important basis for tumor theranostic application. Here, near-infrared (NIR) fluorescent and low-biotoxicity Ag₂Se quantum dots (QDs) have been coupled with cetuximab, a clinical antiepidermal growth factor receptor antibody drug for tumor therapy, via a facile bioconjugation strategy to prepare multifunctional Ag₂Se–cetuximab nanoprobes. Compared with the Ag₂Se QDs alone, the Ag₂Se–cetuximab nanoprobes display faster and more enrichment at the site of orthotopic tongue cancer, and thus present better NIR fluorescence contrast between the tumor and the surrounding regions. At 24 h postinjection, the NIR fluorescence of Ag₂Se–cetuximab nanoprobes at the tumor site is still easily detectable, whereas no fluorescence is observed for the Ag₂Se QDs. Moreover, the Ag₂Se–cetuximab nanoprobes have also significantly inhibited the tumor growth and improved the survival rate of orthotopic tongue cancer-bearing nude mice from 0% to 57.1%. Taken together, the constructed multifunctional Ag₂Se–cetuximab nanoprobes have achieved combined targeted imaging and therapy of orthotopic tongue cancer, which may greatly contribute to the development of nanotheranostics.