Investigation of non-covalent complexations of Ca(II) and Mg(II) ions with insulin by using electrospray ionization mass spectrometry

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• 作者：Mustafa Gü ; lfen ; Abdil Ö ; zdemir ; Jung-Lee Lin and Chung-Hsuan Chen
• 刊名：Rapid Communications in Mass Spectrometry
• 出版年：2016
• 出版时间：15 October 2016
• 年：2016
• 卷：30
• 期：19
• 页码：2171-2182
• 全文大小：4004K
• ISSN：1097-0231

文摘

Insulin is a peptide hormone secreted by pancreatic β-cells. Ca(II) and Mg(II) ions play an important role in the secretion of insulin. There is no study about a direct complexation of Ca(II) or Mg(II) with insulin and their equilibrium constants. Electrospray ionization mass spectrometry (ESI-MS) is a practical method for the monitoring of non-covalent complexes such as Ca(II)-insulin and Mg(II)-insulin. Here, the equilibrium constants of Ca(II)-insulin and Mg(II)-insulin non-covalent complexes have been calculated after ESI-MS measurements in aqueous solutions.