A competitive luminol chemiluminescence immunosensor based on a microfluidic chip for the determination of ractopamine

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• 作者：Sai Wang ; Qilong Chen ; Xiao Wei ; Jian Wu ; Chunyan Wang ; Jiahui Liu ; Liya Zhang and Yiyang Dong
• 刊名：ELECTROPHORESIS
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：38
• 期：2
• 页码：368-371
• 全文大小：273K
• ISSN：1522-2683

文摘

Herein, a competitive luminol chemiluminescence immunosensor based on a microfluidic chip was developed to detect ractopamine (RCT) both in phosphate buffer and swine urine samples. The immunosensor can provide a liner range of 0.5–40 ng/mL and a high sensitivity with a limit of detection of 0.97 ng/mL for RCT detection in swine urine. Good rates of recovery in negative swine urine samples were achieved over the RCT concentration ranging from 0.5 to 40 ng/mL. The proposed method offered a promising analytical scheme for the on-site determination of RCT.