Facilitating best practices in collecting anomalous scattering data for de novo structure solution at the ESRF Structural Biology Beamlines

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• 作者：Daniele de Sanctis ; Marcus Oscarsson ; Alexander Popov ; Olof Svensson and Gordon Leonard
• 关键词：ESRF ; MAD ; experimental phasing ; MXCuBE2
• 刊名：Acta Crystallographica Section D
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：72
• 期：3
• 页码：413-420
• 全文大小：1139K
• ISSN：1399-0047

文摘

The constant evolution of synchrotron structural biology beamlines, the viability of screening protein crystals for a wide range of heavy-atom derivatives, the advent of efficient protein labelling and the availability of automatic data-processing and structure-solution pipelines have combined to make de novo structure solution in macromolecular crystallography a less arduous task. Nevertheless, the collection of diffraction data of sufficient quality for experimental phasing is still a difficult and crucial step. Here, some examples of good data-collection practice for projects requiring experimental phasing are presented and recent developments at the ESRF Structural Biology beamlines that have facilitated these are illustrated.