Comparative analysis of anti-polyglutamine Fab crystals grown on Earth and in microgravity
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- 作者：Gwen E. Owens ; Danielle M. New ; Alejandra I. Olvera ; Julia Ashlyn Manzella ; Brittney L. Macon ; Joshua C. Dunn ; David A. Cooper ; Robyn L. Rouleau ; Daniel S. Connor and Pamela J. Bjorkman
- 关键词：Huntington's disease ; International Space Station ; microgravity ; polyglutamine ; X-ray crystallography ; huntingtin ; crystallization
- 刊名：Acta Crystallographica Section F
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：72
- 期：10
- 页码：762-771
- 全文大小：967K
- ISSN：1744-3091
文摘
Huntington's disease is one of nine neurodegenerative diseases caused by a polyglutamine (polyQ)-repeat expansion. An anti-polyQ antigen-binding fragment, MW1 Fab, was crystallized both on Earth and on the International Space Station, a microgravity environment where convection is limited. Once the crystals returned to Earth, the number, size and morphology of all crystals were recorded, and X-ray data were collected from representative crystals. The results generally agreed with previous microgravity crystallization studies. On average, microgravity-grown crystals were 20% larger than control crystals grown on Earth, and microgravity-grown crystals had a slightly improved mosaicity (decreased by 0.03°) and diffraction resolution (decreased by 0.2 Å) compared with control crystals grown on Earth. However, the highest resolution and lowest mosaicity crystals were formed on Earth, and the highest-quality crystal overall was formed on Earth after return from microgravity.