X-ray radiation damage to biological macromolecules: further insights

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• 作者：Elspeth F. Garman and Martin Weik
• 关键词：X-ray radiation damage ; macromolecular crystallography ; SAXS ; dose ; imaging ; scavengers
• 刊名：Journal of Synchrotron Radiation
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：24
• 期：1
• 页码：1-6
• 全文大小：228K
• ISSN：1600-5775

文摘

Despite significant progress made over more than 15 years of research, structural biologists are still grappling with the issue of radiation damage suffered by macromolecular crystals which is induced by the resultant radiation chemistry occurring during X-ray diffraction experiments. Further insights into these effects and the possible mitigation strategies for use in both diffraction and SAXS experiments are given in eight papers in this volume. In particular, damage during experimental phasing is addressed, scavengers for SAXS experiments are investigated, microcrystals are imaged, data collection strategies are optimized, specific damage to tyrosine residues is reexamined, and room temperature conformational heterogeneity as a function of dose is explored. The brief summary below puts these papers into perspective relative to other ongoing radiation damage research on macromolecules.