The microfluidic bioreactor for a new era of bioprocess development
详细信息 查看全文
- 作者：Christian Blesken ; Till Olfers ; Alexander Grimm and Niklas Frische
- 关键词：Bioprocess development ; Fed-batch ; Microbioreactor ; Microfermentation ; Microfluidic
- 刊名：Engineering in Life Sciences
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：16
- 期：2
- 页码：190-193
- 全文大小：486K
- ISSN：1618-2863
文摘
The BioLector® Pro system from m2p-labs GmbH uses microtiter plates (MTPs) with an integrated microfluidic chip. By using microfluidic technology, the system can successfully carry out small-scale fed-batch cultivations. Working volumes of 0.8–1.5 mL are used to conduct cultivations as they were only as yet possible in lab fermenters. The measurements of biomass, fluorescence, pH and dissolved oxygen are performed by non-invasive optical methods. The control of pH and feeding rates are realized by micro-valves and micro-channels. For the first time, these unique microfluidic components achieve continuous feeding and pH control on an MTP format. Altogether, 32 bioreactor wells and 16 reservoir wells are placed on one plate. That means 32 fed-batch cultivations can be run in parallel, completely automated, with extensive data output.