Mass spectrometry hyphenated techniques for the analysis of volatiles and peptides in soft cheese: Useful tools for the shelf life optimization

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• 作者：Annalisa Mentana ; Anna Natale ; Carmen Palermo ; Donatella Nardiello ; Amalia Conte ; Matteo Alessandro Del Nobile ; Maurizio Quinto and Diego Centonze
• 刊名：ELECTROPHORESIS
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：37
• 期：13
• 页码：1861-1872
• 全文大小：546K
• ISSN：1522-2683

文摘

In order to assess the product quality and shelf life of an Italian soft cream cheese under different storage conditions, the volatile and peptide profiles evolution were tested. Volatiles were sampled directly from the head space of cheese packaging by solid-phase microextraction and analyzed by GC-MS. Peptide profiles were obtained by nanoLC-MS/MS, following a novel bioinformatics approach based on scoring distribution associated to the protein hits originating from the database search. In particular, a refined identification by focusing on selected time segments corresponding to the most intense peaks was carried out. A total of 40 compounds including acids, aldehydes, ketones, lactones, alcohols, esters, hydrocarbons, terpene, sulfur, and aromatic compounds were detected. Significant differences in their abundance during the storage in different packagings were observed, as well as an evolution of peptides mainly belonging to αS1-casein. The results demonstrated the usefulness of the above-mentioned hyphenated techniques for the determination of the soft cheese shelf life under different storage conditions.