Rapid Identification of the Receptor-Binding Specificity of Influenza A Viruses by Fluorogenic Glycofoldamers

详细信息    查看全文

• 作者：Dr. Xiao-Peng He ; Ya-Li Zeng ; Xin-Ying Tang ; Dr. Na Li ; Prof. Dong-Ming Zhou ; Prof. Guo-Rong Chen and Prof. He Tian
• 刊名：Angewandte Chemie
• 出版年：2016
• 出版时间：November 2, 2016
• 年：2016
• 卷：128
• 期：45
• 页码：14201-14205
• 全文大小：3159K
• ISSN：1521-3757

文摘

The re-emergence of influenza raises a global concern that viral pandemics can unpredictably occur. However, effective approaches that can probe the infection risk of influenza viruses for humans are rare. In this work, we develop a glycofoldamer that can rapidly identify the glycan-receptor specificity of influenza viruses in a high-throughput manner. The coupling of glycan receptors that can be recognized by hemagglutinin (a surface protein on the virion capsid of influenza) to a fluorogenic-dye foldamer produces the glycofoldamers with minimal fluorescence in aqueous solution. After interaction with human-infecting virus strains for only five minutes, the fluorescence intensity of the glycofoldamer is remarkably enhanced with a blue-shifted emission peak. The probes have also proven effective for the rapid identification of 1) the human- or bird-infecting properties of influenza viruses in a high-throughput manner and 2) the receptor-specificity switch of a virus strain by mutations.