On page 2636, Dong-Woo Cho and co-workers introduce a skeletal muscle-derived bioink with 3D cell printing technology that facilitates the fabrication of 3D functional muscle constructs. 3D cell printing technology can generate various types of constructs and cellular alignments in a controlled manner. The bioink retains the major ECM molecules that provide a microenvironment similar to that of native muscle and induce myogenesis in 3D cell-printed muscle constructs.