Backfilling-Free Strategy for Biopatterning on Intrinsically Dual-Functionalized Poly[2-Aminoethyl Methacrylate-co-Oligo(Ethylene Glycol) Methacrylate] Films
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- 作者：Dr. Bong Soo Lee ; Dr. Juno Lee ; Gyeongyeop Han ; EunRae Ha ; Prof. Dr. Insung S. Choi and Prof. Dr. Jungkyu K. Lee
- 刊名：Chemistry – An Asian Journal
- 出版年：2016
- 出版时间：July 20, 2016
- 年：2016
- 卷：11
- 期：14
- 页码：2057-2064
- 全文大小：1186K
- ISSN：1861-471X
文摘
We demonstrated protein and cellular patterning with a soft lithography technique using poly[2-aminoethyl methacrylate-co-oligo(ethylene glycol) methacrylate] films on gold surfaces without employing a backfilling process. The backfilling process plays an important role in successfully generating biopatterns; however, it has potential disadvantages in several interesting research and technical applications. To overcome the issue, a copolymer system having highly reactive functional groups and bioinert properties was introduced through a surface-initiated controlled radical polymerization with 2-aminoethyl methacrylate hydrochloride (AMA) and oligo(ethylene glycol) methacrylate (OEGMA). The prepared poly(AMA-co-OEGMA) film was fully characterized, and among the films having different thicknesses, the 35 nm-thick biotinylated, poly(AMA-co-OEGMA) film exhibited an optimum performance, such as the lowest nonspecific adsorption and the highest specific binding capability toward proteins.