An Improved PCR-RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene
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- 作者：Xiaolei Feng ; Sihua Wang ; Xiaoran Duan ; Chunyang Li ; Zhen Yan ; Feifei Feng ; Songcheng Yu ; Yongjun Wu and Wei Wang
- 刊名：Journal of Clinical Laboratory Analysis
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：30
- 期：6
- 页码：986-989
- 全文大小：2655K
- ISSN：1098-2825
文摘
In recent research, it has been shown that rs2289487 within the PLIN1 gene has different variants that have been associated with obesity, type 2 diabetes, and other diseases. However, the isochizomers such as the BsmI enzyme required for detection of this polymorphism through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method are expensive. In this study, we aimed to explore a novel PCR-RFLP method for identifying the single-nucleotide polymorphism (SNP) of rs2289487 of PLIN1 gene.