Separation of three anthraquinone glycosides including two isomers by preparative high-performance liquid chromatography and high-speed countercurrent chromatography from Rheum tanguticum Maxim. ex Balf

详细信息    查看全文

• 作者：Tao Chen ; Hongmei Li ; Denglang Zou ; Yongling Liu ; Chen Chen ; Guoying Zhou and Yulin Li
• 刊名：Journal of Separation Science
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：39
• 期：16
• 页码：3105-3112
• 全文大小：3581K
• ISSN：1615-9314

文摘

Anthraquinone glycosides, such as chrysophanol 1-O-β-d-glucoside, chrysophanol 8-O-β-d-glucoside, and physion 8-O-β-d-glucoside, are the accepted important active components of Rheum tanguticum Maxim. ex Balf. due to their pharmacological properties: antifungal, antimicrobial, cytotoxic, and antioxidant activities. However, an effective method for the separation of the above-mentioned anthraquinone glycosides from this herb is not currently available. Especially, greater difficulty existed in the separation of the two isomers chrysophanol 1-O-β-d-glucoside and chrysophanol 8-O-β-d-glucoside. This study demonstrated an efficient strategy based on preparative high-performance liquid chromatography and high-speed countercurrent chromatography for the separation of the above-mentioned anthraquinone glycosides from Rheum tanguticum Maxim.ex Balf.