MicroRNA‐17 regulates autophagy to promote hepatic ischemia/reperfusion injury via suppression of signal transductions and activation of transcription‐3 expression

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• 作者：Shipeng Li ; Jianjun Zhang ; Zhen Wang ; Tengfei Wang ; Yao Yu ; Jindan He ; Haiming Zhang ; Tao Yang ; Zhongyang Shen
• 刊名：Liver Transplantation
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：22
• 期：12
• 页码：1697-1709
• 全文大小：948.0KB
• ISSN：1527-6473

文摘

Hepatic ischemia/reperfusion injury (IRI) represents an important clinical problem as related to liver resection or transplantation. However, the potential mechanism underlying hepatic IRI remains obscure. Recent evidence has indicated that microRNAs (miRNAs) participate in various hepatic pathophysiological processes via regulating autophagy. This relationship between MicroRNA-17 (miR-17) and hepatic autophagy prompted us to examine the role and potential mechanisms of miR-17 regulating autophagy in hepatic IRI. MiR-17 levels were significantly up-regulated after hepatic ischemia/reperfusion (IR), and the number of autophagosomes increased in response to IR. These results demonstrate that miR-17 could promote hepatic IRI as revealed by reductions in cell viability in vitro. The expression of microtubule-associated protein 1 light B II (LC3BII) was gradually up-regulated and peaked at 24 hours following reperfusion, a time point that was also associated with maximal miR-17 levels. Overexpression of miR-17 diminished signal transductions and activation of transcription-3 (Stat3) and phosphorylated Stat3 (p-Stat3) levels, an effect which promoted autophagy in response to IRI. However, low-level expressions of miR-17 were associated with increased Stat3 and p-Stat3 levels and decreased autophagy. In conclusion, high levels of miR-17 expression can function to up-regulate autophagy to aggravate hepatic IRI by suppressing Stat3 expression. Liver Transplantation 22 1697–1709 2016 AASLD.