An Iridium(III) Complex as a Photoactivatable Tool for Oxidation of Amyloidogenic Peptides with Subsequent Modulation of Peptide Aggregation
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- 作者：Juhye Kang ; Dr. Shin Jung C. Lee ; Jung Seung Nam ; Dr. Hyuck Jin Lee ; Myeong-Gyun Kang ; Kyle J. Korshavn ; Hyun-Tak Kim ; Prof. Dr. Jaeheung Cho ; Prof. Dr. Ayyalusamy Ramamoorthy ; Prof. Dr. Hyun-Woo Rhee ; Prof. Dr. Tae-Hyuk Kwon and Prof. Dr. Mi Hee Lim
- 刊名：Chemistry - A European Journal
- 出版年：2017
- 出版时间：January 31, 2017
- 年：2017
- 卷：23
- 期：7
- 页码：1645-1653
- 全文大小：3740K
- ISSN：1521-3765
文摘
Aggregates of amyloidogenic peptides are involved in the pathogenesis of several degenerative disorders. Herein, an iridium(III) complex, Ir-1, is reported as a chemical tool for oxidizing amyloidogenic peptides upon photoactivation and subsequently modulating their aggregation pathways. Ir-1 was rationally designed based on multiple characteristics, including 1) photoproperties leading to excitation by low-energy radiation; 2) generation of reactive oxygen species responsible for peptide oxidation upon photoactivation under mild conditions; and 3) relatively easy incorporation of a ligand on the IrIII center for specific interactions with amyloidogenic peptides. Biochemical and biophysical investigations illuminate that the oxidation of representative amyloidogenic peptides (i.e., amyloid-β, α-synuclein, and human islet amyloid polypeptide) is promoted by light-activated Ir-1, which alters the conformations and aggregation pathways of the peptides. Additionally, their potential oxidation sites are identified as methionine, histidine, or tyrosine residues. Overall, our studies on Ir-1 demonstrate the feasibility of devising metal complexes as chemical tools suitable for elucidating the nature of amyloidogenic peptides at the molecular level, as well as controlling their aggregation.