A Novel Magnetism-assisted Electrochemical Immunosensor with Sub-Picomolar Sensitivity
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- 作者：Pengli Li and Hongfang Zhang
- 刊名：Journal of the Chinese Chemical Society
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：63
- 期：9
- 页码：784-791
- 全文大小：474K
- ISSN：2192-6549
文摘
A novel electrochemical immunosensor based on a magnetic glassy carbon electrode (MGCE) was developed for the quantitative determination of human immunoglobulin G (IgG). The immunosensing interface was fabricated by initially depositing silver nanoparticles on the MGCE surface and then immobilizing anti-human IgG antibodies via the magnetic force between MGCE and Fe3O4 nanoparticles. The antibodies were covalently bonded to the amine-functionalized Fe3O4 nanoparticles. Under optimal conditions, the magnetism-assisted immunosensor exhibited a wide linear range from 0.1 pg/mL to 1.0 µg/mL with the detection limit of 0.05 pg/mL. Furthermore, the immunosensor displayed the advantages of good reproducibility and satisfactory stability.