Fine mapping of the R_SC8 locus and expression analysis of candidate SMV resistance genes in soybean

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• 作者：Lin Zhao ; Dagang Wang ; Hongyun Zhang ; Yingchao Shen ; Yongqing Yang ; Kai Li ; Liqun Wang ; Yunhua Yang and Haijian Zhi
• 刊名：Plant Breeding
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：135
• 期：6
• 页码：701-706
• 全文大小：473K
• ISSN：1439-0523

文摘

Soybean mosaic disease, caused by soybean mosaic virus (SMV), is one of the most broadly distributed viral diseases of soybean (Glycine max [L.] Merr.) worldwide, which decreases seed quality and causes serious losses in yields. The accession ‘Kefeng No.1’ was previously shown to carry the R_SC8 gene on chromosome 2, which confers resistance to SMV strain SC8. To fine map the R_SC8 gene, we generated larger F₂ population (2122 plants) from the cross between resistant accession ‘Kefeng No.1’ and susceptible variety ‘Nannong 1138-2’. Using eight genomic-SSR markers including four newly developed markers, we mapped this gene to a 30.8-kb region containing two annotated genes between two markers, ZL-42 and ZL-52, on chromosome 2. The expression of genes Glyma.02G121500 and Glyma.02G121600 (encoding MADS-box proteins) was higher in ‘Kefeng No.1’ plants than that in ‘Nannong 1138-2’ plants, as determined by quantitative reverse transcription–polymerase chain reaction (QRT-PCR). These results suggest that the two genes are likely involved in conferring SMV resistance. This study will facilitate marker-assisted selection of this gene for use in soybean breeding programmes.