Development of a rapid 21-plex autosomal STR typing system for forensic applications

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• 作者：Meng Yang ; Caiyong Yin ; Yuexin Lv ; Yaran Yang ; Jing Chen ; Zailiang Yu ; Xu Liu ; Meibo Xu ; Feng Chen ; Huijuan Wu and Jiangwei Yan
• 刊名：ELECTROPHORESIS
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：37
• 期：21
• 页码：2789-2799
• 全文大小：1001K
• ISSN：1522-2683

文摘

DNA-STR genotyping technology has been widely used in forensic investigations. Even with such success, there is a great need to reduce the analysis time. In this study, we established a new rapid 21-plex STR typing system, including 13 CODIS loci, Penta D, Penta E, D12S391, D2S1338, D6S1043, D19S433, D2S441 and Amelogenin loci. This system could shorten the amplification time to a minimum of 90 min and does not require DNA extraction from the samples. Validation of the typing system complied with the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Chinese National Standard (GA/T815-2009) guidelines. The results demonstrated that this 21-plex STR typing system was a valuable tool for rapid criminal investigation.