Derivatization coupled to headspace programmed-temperature vaporizer gas chromatography with mass spectrometry for the determination of amino acids: Application to urine samples

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• 作者：Rosa Mar&iacute ; a Gonzá ; lez Paredes ; Carmelo Garc&iacute ; a Pinto ; José ; Luis Pé ; rez Pavó ; n and Bernardo Moreno Cordero
• 刊名：Journal of Separation Science
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：39
• 期：17
• 页码：3375-3383
• 全文大小：4211K
• ISSN：1615-9314

文摘

A new method based on headspace programmed-temperature vaporizer gas chromatography with mass spectrometry has been developed and validated for the determination of amino acids (alanine, sarcosine, ethylglycine, valine, leucine, and proline) in human urine samples. Derivatization with ethyl chloroformate was employed successfully to determine the amino acids. The derivatization reaction conditions as well as the variables of the headspace sampling were optimized.