Voltammetric Determination of Tumor Biomarkers for Neuroblastoma (Homovanillic Acid, Vanillylmandelic Acid, and 5-Hydroxyindole-3-acetic Acid) at Screen-printed Carbon Electrodes
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- 作者：Anna Makrlí ; ková ; Evangelia Ktena ; Anastasios Economou ; Jan Fischer ; Tomá ; š Navrá ; til ; Jiří ; Barek and Vlastimil Vyskočil
- 刊名：Electroanalysis
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：29
- 期：1
- 页码：146-153
- 全文大小：1682K
- ISSN：1521-4109
文摘
Three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA)) have been determined by differential pulse voltammetry (DPV) at screen-printed carbon electrodes (SPCEs). As an optimum medium for all three analytes, Britton-Robinson buffer (0.04 mol ⋅ L<sup>−1sup>, pH 3.0) was used. Dependencies of the peak current on the concentration of a biomarker were linear in the concentration range from 0.2 to 100 μmol ⋅ L<sup>−1sup> for all the tested compounds, with the limits of quantification 0.8 μmol ⋅ L<sup>−1sup> for homovanillic acid, 0.2 μmol ⋅ L<sup>−1sup> for vanillylmandelic acid, and 0.4 μmol ⋅ L<sup>−1sup> for 5-hydroxyindole-3-acetic acid. Moreover, it was verified that the newly developed methods can be used for simultaneous determination of HVA and VMA in mixture.