Cell Surface Nano-modulation for Non-invasive in vivo Near-IR Stem Cell Monitoring
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- 作者：Woo Jung Shin ; Seung Won Shin ; Ji Soo Yuk ; Dr. Lunjakorn Amornkitbamrung ; Min Su Jang ; In Hyun Song ; Dr. Soon Won Choi ; Insung Kang ; Jin Young Lee ; Prof. Dr. Hojae Bae ; Prof. Dr. Kyung-Sun Kang and Prof. Dr. Soong Ho Um
- 刊名：ChemMedChem
- 出版年：2017
- 出版时间：January 5, 2017
- 年：2017
- 卷：12
- 期：1
- 页码：28-32
- 全文大小：1296K
- ISSN：1860-7187
文摘
A stem cell tracking system is in high demand for the determination of cell destinations and for the validation of cell therapeutic efficacy in regenerative transplantation. To date, near-infrared (NIR) imaging technology has received considerable attention in cell behavior monitoring, owing to its patient compatibility, easy accessibility and cost effectiveness. Conventionally, in vivo cell tracking has been visualized by direct in-cell staining with NIR, where it may be achieved by complicated genetic engineering. Such genetic amendment techniques have suffered from serious challenges, which can destroy a cell's metabolism and can accidentally incur unexpected carcinoma. Herein we demonstrate a novel cell nano-modulation method for noninvasive stem cell monitoring. It is simply achieved by conjugating stem cells with lipid-supported, NIR-tagged, polymeric nanoparticles. These engineered cells, which are designated as NIR-labeled light-emitting stem cells (LESCs), maintain their biochemical functionality (i.e., differentiation, quantum efficacy, etc.) even after conjugation. LESCs were used for in situ stem cell monitoring at inoculation sites. It is speculated that the LESC technique could provide a new preparative methodology for in vivo cell tracking in advanced diagnostic medicine, where cell behavior is a critical issue.