Respiratory viruses associated with severe pneumonia in children under 2 years old in a rural community in Pakistan

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• 作者：Asad Ali ; Tauseef Akhund ; Gohar Javed Warraich ; Fatima Aziz ; Najeeb Rahman ; Fayyaz Ahmed Umrani ; Shahida Qureshi ; William A. Petri Jr. ; Zulfiqar Bhutta ; Anita K. M. Zaidi and Molly A. Hughes
• 刊名：Journal of Medical Virology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：88
• 期：11
• 页码：1882-1890
• 全文大小：553K
• ISSN：1096-9071

文摘

The objective of this study was to determine the incidence of respiratory viruses associated with severe pneumonia among children less than 2 years of age in the rural district of Matiari in Sindh, Pakistan. This study was a community-based prospective cohort active surveillance of infants enrolled at birth and followed for 2 years. Cases were identified using the World Health Organization's Integrated Management of Childhood Illnesses’ definition of severe pneumonia. Nasopharyngeal swabs were obtained for assessment by multiplex RT-PCR for eight viruses and their subtypes, including RSV, influenza virus, human metapneumovirus, enterovirus/rhinovirus, coronavirus, parainfluenza virus, adenovirus, and human bocavirus. Blood cultures were collected from febrile participants. A total of 817 newborns were enrolled and followed with fortnightly surveillance for 2 years, accounting for a total of 1,501 child-years of follow-up. Of the nasopharyngeal swabs collected, 77.8% (179/230) were positive for one or more of the above mentioned respiratory viruses. The incidence of laboratory confirmed viral-associated pneumonia was 11.9 per 100 child-years of follow-up. Enterovirus/rhinovirus was detected in 51.7% patients, followed by parainfluenza virus type III (8.3%), and RSV (5.7%). Of the uncontaminated blood cultures, 1.4% (5/356) were positive. Respiratory viruses are frequently detected during acute respiratory infection episodes in children under 2 years old in a rural community in Pakistan. However, causal association is yet to be established and the concomitant role of bacteria as a co-infection or super-infection needs further investigation. J. Med. Virol. 88:1882–1890, 2016.