Allergen-specific immune response suppresses interleukin 10 expression in B cells via increasing micro-RNA-17-92 cluster

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• 作者：Xiao-Rui Geng ; Shu-Qi Qiu ; Li-Tao Yang ; Zhi-Qiang Liu ; Gui Yang ; Jiang-Qi Liu ; Lu Zeng ; Xiao-Xi Li ; Li-Hua Mo ; Zhi-Gang Liu and Ping-Chang Yang
• 刊名：Cell Biochemistry and Function
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：34
• 期：6
• 页码：449-454
• 全文大小：249K
• ISSN：1099-0844

文摘

Interleukin (IL)-10–expressing B cells play a critical role in the immune homeostasis in the body; its regulation has not been fully understood. Micro-RNA (miR)-17-92 cluster has strong regulation in the immunity. This study tests a hypothesis that miR-17-92 cluster suppresses IL-10 expression in B cells. In this study, peripheral B cells were collected from patients with allergic rhinitis (AR). The B cells were treated with specific allergens, dust mite extracts, in the culture. The expressions of miR-17-92 cluster and IL-10 in the culture were assessed by real-time quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. The results showed that the levels of miR-19a, but not the rest of the 5 members (miR-17, miR-18a, miR-19b, miR-20a, and miR-92a), were significantly higher in peripheral B cells from AR patients as than in B cells from healthy participants. Exposure of B cells from AR patients to specific allergen, dust mite extracts, significantly increased the levels if miR-19a and suppressed the expression of IL-10 in B cells. The levels of histone deacetylase 11 and acetylated H3K9 were higher, and the RNA polymerase II and c-Maf (the IL-10 transcription factor) were lower, at the IL-10 promoter locus. In conclusion, miR-19a mediates the allergen-specific immune response–decreased IL-10 expression in B cells.