Increased survival of human free fat grafts with varying densities of human adipose-derived stem cells and platelet-rich plasma

详细信息    查看全文

• 作者：Kun Li ; Feng Li ; Jie Li ; Hang Wang ; Xiaohui Zheng ; Jie Long ; Weihua Guo and Weidong Tian
• 刊名：Journal of Tissue Engineering and Regenerative Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：11
• 期：1
• 页码：209-219
• 全文大小：642K
• ISSN：1932-7005

文摘

The high absorption rate of transplanted fat has limited the application of autogenous fat grafts in the clinical setting. Therefore, this study aimed to evaluate the effects of platelet-rich plasma (PRP) and adipose-derived stem cells (ASCs) on fat regeneration by investigating the impact of PRP and conditioned medium on the biological characteristics of ASCs. Fat grafts were prepared with ASCs at densities of 10⁷/ml, 10⁶/ml, 10⁵/ml, 10⁴/ml and 0/ml with and without PRP and injected subcutaneously into nude mice. Liquid overflow method, haematoxylin and eosin staining, and immunohistochemical analyses were used to examine the fat grafts. The residual fat volume of the 10⁵/ml ASC + PRP group was significantly higher than that of other treatment conditions after 90 days. Furthermore, histological examination revealed that in 10⁵/ml ASCs-treated grafts normal adipocyte area and capillary formation were increased dramatically compared with other treatment conditions. It is concluded that fat grafts consisting of PRP and 10⁵/ml ASCs constitute an ideal transplant strategy, which may result in decreased absorption and accelerated fat regeneration. This simple and reliable method could provide a valuable and needed tool in plastic and reconstructive surgery. Copyright