HPLC Analysis of Twelve Compounds from Artemisia sacrorum Ledeb and their Structural Elucidation
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- 作者：Wugedunqiqige Bao ; Qinghu Wang ; Jingjing Han and Narenchaoketu Han
- 刊名：Journal of Food Biochemistry
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：41
- 期：1
- 全文大小：135K
- ISSN：1745-4514
文摘
An investigation of ethyl acetate extracts from Artemisa sacrorum Ledeb (A. sacrorum) has led to the isolation of two new flavonoids together with 10 known compounds. At the same time, a high-performance liquid chromatographic method is described for the simultaneous determination of the 12 compounds in A. sacrorum. The separation by gradient elution was performed on Hypersil ODS-2 column (250 mm × 4.6 mm, 5 μm) at 30C with acetonitrile and water as the mobile phase, and monitored by absorbance at 261 nm. The recovery of the method is from 93.33 to 98.67%, and linearity (r > 0.9992) was obtained for 12 compounds. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.0%) were also achieved. This assay was successfully applied to the determination of 12 compounds in 10 samples.