Biological activity of peptide-conjugated polyion complex matrices consisting of alginate and chitosan

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• 作者：Chikara Fujimori ; Jun Kumai ; Kyotaro Nakamura ; Yingzi Gu ; Fumihiko Katagiri ; Kentaro Hozumi ; Yamato Kikkawa and Motoyoshi Nomizu
• 刊名：Peptide Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：108
• 期：1
• 全文大小：1207K
• ISSN：1097-0282a

文摘

Peptide-conjugated polysaccharide matrices using bioactive laminin-derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide-polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde-alginate was synthesized by oxidization of alginate using NaIO₄, and then, reacted with Cys-peptides. Next, the peptide-alginate solution was added to a chitosan-coated plate, and the peptide-polyion complex matrices (peptide-PCMs) were prepared. The peptide-PCMs using an integrin αvβ3-binding peptide (A99a: ALRGDN, mouse laminin α1 chain 1145-1150) and an integrin α2β1-binding peptide (EF1XmR: RLQLQEGRLHFXFD, X = Nle, mouse laminin α1 chain 2751-2763) showed strong cell attachment activity in a dose-dependent manner. When we examined the effect of various spacers on the biological activity of A99a-PCM, hydrophobic and long spacers enhanced the cell attachment activity. Further, the A99a-PCM with the spacers strongly promoted neurite outgrowth. The polyion complex method is an easy way to obtain insolubilized matrix and is widely applicable for various polysaccharides. The peptide-PCM is useful as a biomaterial for cell and tissue engineering.