Protective Mechanisms of Unsaponifiable Matter from Rice Bran Against Tert-Butyl Hydroperoxide-Induced Oxidative Damage in HepG2 Cells

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• 作者：Hyeonmi Ham ; Yu Young Lee ; Ji-Young Park ; Choonwoo Lee ; Jieun Kwak ; In-Hwan Kim and Junsoo Lee
• 刊名：Journal of Food Biochemistry
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：40
• 期：4
• 页码：526-534
• 全文大小：372K
• ISSN：1745-4514

文摘

Rice bran is rich source of phytochemicals, such as phytosterols, tocopherols, tocotrienols, policosanols, γ-oryzanol, triterpene alcohol and other minor compounds. In this study, we investigated the mechanisms underlying the protective effects of unsaponifiable matter from rice bran on tert-butyl hydroperoxide (TBHP)-induced oxidative stress in HepG2 cells. HepG2 cells were pretreated with different concentrations of USM (0–100 µg/mL) for 6 h and oxidative stress was induced by TBHP. The result indicated that pretreatment of HepG2 cells with USM significantly reduced TBHP-induced oxidative damage, as determined by cell cytotoxicity and intracellular reactive oxygen species generations. USM activated nuclear factor-erythroid 2-related factor 2 (Nrf2) nuclear translocation and subsequently enhanced the expression of phase II detoxifying enzymes including heme oxygenase-1, glutamate-cysteine ligase catalytic subunit, and NADPH: quinine oxidoreductase-1. These results suggest that USM-mediated up-regulation of phase II detoxifying enzymes via Nrf2-signaling pathways may provide a pivotal mechanism for its hepatoprotective action.