Fontibacillus pullulanilyticus sp.nov. isolated from soil

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• 作者：Kadriye Inan Bektas ; Ali Osman Belduz ; Hatice Korkmaz Guvenmez and Damla Sihay
• 刊名：Journal of Basic Microbiology
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：56
• 期：8
• 页码：857-863
• 全文大小：168K
• ISSN：1521-4028

文摘

Gram stain-negative, motile, catalase-, and oxidase- positive strain, designated DSHK107^T was isolated from soil of Cukurova University campus in Adana, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain grew at 20–42 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in 0–2.0% NaCl (w/v). m>16S rRNAm> gene sequence analysis revealed that the strain belonged to the genus m>Fontibacillusm>; strain DSHK107^T showed highest sequence similarity to type strains m>Fontibacillus aquaticusm> GPTSA 19^T (97.8%) and m>Fontibacillus panacisegetism> P11-6^T (97.0%). The major fatty acid of strain DSHK107^T was anteiso-C_15:0 (46.7). The polar lipids of strain DSHK107^T consisted of dihosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unknown phospholipids, four unknown lipids, three unknown glycolipids, two unknown aminophospholipids, an unknown aminolipid, and an unknown aminophosphoglycolipid. The major isoprenoid quinone was MK-7. The DNA G+C content of DSHK107^T was 42.1 mol%. DNA–DNA hybridization showed that the strain DSHK107^T shared low DNA–DNA relatedness with m>F. aquaticusm> DSM 17643^T, m>Fontibacillus solanim> A4STRO4^T, and m>F. panacisegetism> DSM 28129^T (47, 58, and 59.3%, respectively). Thus, our results support the placement of strain DSHK107^T within a separate and previously unrecognized species. On the basis of a taxonomic study using a polyphasic approach, strain DSHK107^T is considered to represent a novel species of the genus m>Fontibacillusm>, for which the name m>Fontibacillus pullulanilyticusm> sp. nov. is proposed. The type strain is DSHK107^T (=NCCB 100560^T = DSM 100116^T).