Bioguided Isolation and Antiproliferative Activity of Constituents from Smilax korthalsii A.D.C. Leaves

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• 作者：Abdulmumeen A. Hamid ; Olapeju O. Aiyelaagbe ; Arvind S. Negi ; Suaib Luqman and Fatima Kaneez
• 刊名：Journal of the Chinese Chemical Society
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：63
• 期：7
• 页码：562-571
• 全文大小：234K
• ISSN：2192-6549

文摘

Ethyl acetate extract of Smilax korthalsii A.D.C. leaves exhibited antiproliferative activity on leukaemia carcinoma K562, hepatic liver cancer cells WRL and colorectal carcinoma with IC₅₀ of 125.20, 46.10 and 160.00 μM respectively. Isolation of the bioactive ethyl acetate extract of Smilax korthalsii A.D.C. leaves gave eight compounds; 3β-hydroxyspirost-5-ene (diosgenin), 1, β-sitosterol, 2, lup-5,11,20-trien-23-ol, 3, uneicos-9-enoic acid, 4, ethylheptadecan-17-oic-9-enoate, 5, cis-octadec-9-enoic acid, 6, hexadec9-enoic acid, 7 and 11-methyltridec-12-en-1-ol, 8. The isolated compounds were tested against four human cancer cell lines: leukaemia carcinoma, K-562; hepatic liver cancer cells, WRL; colorectal carcinoma, COLO; and breast carcinoma, MCF-7 using the MTT assay. Diosgenin 1 exhibited significant antiproliferative activity against all four cell lines (IC₅₀; K562=6.25, WRL=14.34, COLO=38.00, MCF-7=12.40 μM), while compounds 3, 6 and 7 inhibited the growth of K-562 at 20, 50 and 100 μM concentrations with IC₅₀ of 90.20, 75.92 and 50.72 μM respectively. Other isolated compounds also showed cytotoxic properties against K-562, WRL and COLO, but showed low inhibition of MCF-7.