Enzymatic N-Glycosylation of Diverse Arylamine Aglycones by a Promiscuous Glycosyltransferase from Carthamus tinctorius

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• 作者：Kebo Xie ; Ridao Chen ; Dawei Chen ; Jianhua Li ; Ruishan Wang ; Lin Yang and Jungui Dai
• 刊名：Advanced Synthesis & Catalysis
• 出版年：2017
• 出版时间：February 20, 2017
• 年：2017
• 卷：359
• 期：4
• 页码：603-608
• 全文大小：1026K
• ISSN：1615-4169

文摘

A new glycosyltransferase (UGT71E5) from Carthamus tinctorius exhibited a robust promiscuity towards 30 structurally diverse drug-like aromatic amine scaffolds, making it the first reported glycosyltransferase capable of catalyzing N-glycosylation with multiple diverse nitrogen-heterocyclic aromatic compounds. The catalytic promiscuity and reversibility of UGT71E5 was exploited to generate the rare N-glycoside from the abundant O-glycoside with high efficiency. These findings demonstrate the significant potential of UGT71E5 in the enzymatic synthesis of diverse bioactive N-glycosides.