Quality Assessment of Two Spectrophotometric Procedures for Polyphenol Determination and Application in Moltkia petraea Species

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• 作者：Jadranka Vuković Rodrí ; guez ; Renata Jurišić Grubešić ; Dario Kremer and Virna Kokot
• 刊名：Journal of the Chinese Chemical Society
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：63
• 期：8
• 页码：677-687
• 全文大小：146K
• ISSN：2192-6549

文摘

A comparison study of quality of two spectrophotometric procedures: Schneider’s (T-Sch) and 4-aminoantipyrine (T-AAP) procedures for total polyphenols determination was carried out using informative prevalidation strategy. Good quality of the measurements at the lower analyte level, excellent resolution of signals, homogenous data material, ideal linear calibration and analytical evaluation functions, very low limit of quantitation (0.13 μg/mL), and high precision (from ±0.60 % to ±3.43 %) and accuracy (from −4.26 % to +4.00 %) confirmed the high quality of the T-Sch procedure. Although favorable performance characteristics of T-AAP procedure were obtained, the signal resolution, sensitivity, precision and accuracy are somewhat lower than in T-Sch procedure. Both prevalidated procedures were successfully applied to determine total polyphenols in leaves, stems, and flowers in three populations of endemic IllyricBalkan species Moltkia petraea (Tratt.) Griseb. growing in Croatia. Total concentrations of polyphenols obtained by T-Sch procedure in all plant organs of all populations were somewhat lower than determined by T-AAP procedure. However, the relative abundance of polyphenols obtained by both methods is the same. Prevalidation results demonstrated the reliability of the T-AAP and T-Sch procedures as valuable tools in polyphenols analysis and their application provided new information regarding phytochemical characterization of endemic M. petraea.