Autofluorescent gelatin nanoparticles as imaging probes to monitor matrix metalloproteinase metabolism of cancer cells

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• 作者：Bo Cai ; Lang Rao ; Xinghu Ji ; Lin-Lin Bu ; Zhaobo He ; Da Wan ; Yi Yang ; Wei Liu ; Shishang Guo and Xing-Zhong Zhao
• 刊名：Journal of Biomedical Materials Research Part A
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：104
• 期：11
• 页码：2854-2860
• 全文大小：655K
• ISSN：1552-4965

文摘

In this paper, autofluorescent gelatin nanoparticles were synthesized as matrix metalloproteinase (MMP) responsive probes for cancer cell imaging. A modified two-step desolvation method was employed to generate these nanoparticles whose size was controllable and had stable autofluorescence. As glutaraldehyde was introduced as the crosslinking agent, the generation of Schiff base (CN) and double carbon bond (CC) between glutaraldehyde and gelatin endowed these gelatin nanoparticles distinct autofluorescence. Considering MMPs were usually overexpressed on the surface of cancer cells and they had degradation ability toward gelatin, we utilized these nanoparticles as imaging probes to responsively monitor the MMP metabolism of cancer cells according to the luminance change. As fluorescent probes, these nanoparticles had facile synthesis procedure and good biocompatibility, and provided a smart strategy to monitor cancer cell behaviors.