Gene cloning of a novel fungal immunomodulatory protein from Chroogomphis rutilus and its expression in Pichia pastoris
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- 作者：Jing-wei Lin ; Shan-yue Guan ; Zuo-wen Duan ; Yu-hua Shen ; Wen-li Fan ; Li-jing Chen ; Li Zhang ; Liang Zhang and Tian-lai Li
- 刊名：Journal of Chemical Technology & Biotechnology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：91
- 期：11
- 页码：2761-2768
- 全文大小：741K
- ISSN：1097-4660
文摘
Fungal immunomodulatory proteins (FIPs) are a newly identified protein family that exhibits immunomodulation and antitumor activities. However, little is known about the FIPs from Gomphidiaceae species. In this study, a novel FIP gene termed FIP-cru was cloned from Chroogomphis rutilus, analyzed, and functionally expressed in Pichia pastoris GS115. In addtion, the bioactivity of rFIP-cru was examined including hemagglutination, cytotoxicity, and interleukin-2 assays in vitro.