A weighted method for estimation of receptor occupancy for pharmacodynamic measurements in drug development
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- 作者：Ola Sternebring ; Lene Alifrangis ; Toke Folke Christensen ; Hong Ji ; Anne Charlotte Hegelund and Carl-Magnus Hö ; gerkorp
- 关键词：receptor occupancy ; pharmacodynamics ; flow cytometry ; biomarker ; clinical trial ; drug development
- 刊名：Cytometry Part B: Clinical Cytometry
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：90
- 期：2
- 页码：220-229
- 全文大小：378K
- ISSN：1552-4957
文摘
Flow cytometry-based receptor occupancy (RO) assessments for pharmacodynamic (PD) response measurements along with drug pharmacokinetic (PK) measurements represent a cornerstone in mechanism based PK/PD modeling of drugs against cell surface targets. This report describes the utility of using a “Free” and a “Bound” assay in combination to derive RO estimations through a weighted calculation method.