文摘
Bacterial counts of 170 radish sprout (Raphanus sativus) retailed in Japan between June 2012 and February 2013 was examined. Aerobic plate counts and coliform counts exhibited seasonal variations. No Shiga toxin-producing Escherichia coli or Salmonella spp. were detected from them. 16S rRNA pyrosequencing analysis of 16 representative samples from one farm (farm D) revealed a predominance of Pseudomonas spp. throughout seasons, and summer samples exhibited an increase in Enterobacteriaceae including Escherichia spp. and decreases in Oxalobacteraceae (primarily Duganella and Janthinobacterium spp.) and Flavobacteriaceae (Flavobacterium spp.) compared with winter samples. Quantitative-PCR analysis of 40 samples from the farm D confirmed the seasonal dynamics of E. coli and Flavobacterium spp. Radish seeds increased proportion of Pseudomonas spp. after sprouting, compared with pre-sprouted seeds, suggesting that increased proportion of Pseudomonas spp. in retailed samples might be sourced from their seeds. Our data thus provides that atmospheric control at postsprouting stage might be hygienic practical point to secure microbial safety.