Experimental and computational examination of anastellin (FnIII1c)-polymer interactions

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• 作者：David Mallinson ; David L. Cheung ; Dorin Simionesie ; Alexander B. Mullen ; Zhenyu J. Zhang and Dimitrios A. Lamprou
• 刊名：Journal of Biomedical Materials Research Part A
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：105
• 期：3
• 页码：737-745
• 全文大小：789K
• ISSN：1552-4965

文摘

Using a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. Anastellin and superfibronectin have been seen to exhibit antiangiogenic properties and other properties that may make it suitable for consideration for incorporation into biomaterials. The molecular interaction was directly quantified by atomic force microscope (AFM)-based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). Using AFM, it was found that the anastellin molecule facilitates a stronger adhesion on polyurethane films (72.0 pN nm⁻¹) than on poly (methyl methacrylate) films (68.6 pN nm⁻¹). However, this is not consistent with the QCM adsorption measurements, which show no significant difference. Molecular dynamics simulations of the behavior of anastellin on polyurethane in aqueous solution were performed to rationalize the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water.